Experimental evaluation of the influence of gold nanoparticles and cerium dioxide on normal and malignant cells and tissues

Volume 4, Issue 3, June 2020     |     PP. 44-54      |     PDF (160 K)    |     Pub. Date: August 12, 2020
DOI:    215 Downloads     5872 Views  

Author(s)

Reznikov A.G., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Faliush O.A., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Nosenko N.D., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Sachynska O.V., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Polyakova L.I., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Limareva A.A., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine
Perchyk I.G., V.P. Komisarenko Institute of Endocrinology and Metabolism, National Academy of Medical Sciences of Ukraine, 69 Vyshgorodska str., Kiev, 04114, Ukraine

Abstract
Presented are the results of experimental studies of the effect of gold nanoparticles (AuNP) on reproductive organs of male rats, human cells and tissues of androgen-dependent prostate cancer (PCa), as well as effect of cerium dioxide nanoparticles (CeO2NP) on the testes and fertility in aging male rats. Presence of polydisperse colloidal solution of AuNP (10-50 nm) in the LNCaP culture medium at a final concentration of 10 μg/ml inhibited cell growth, while the monodisperse solution of AuNP (20 nm) had no effect. The polydisperse colloidal AuNP solution arrested the growth of human PCa xenografts in mice, when administered parenterally at a dose range of 0.64-6.4 μg/kg b.w. The selectivity of the the nanopreparations effect on the prostate malignant epithelium is confirmed by its destruction and a decrease in the epithelial-stromal ratio on histological preparations of the xenografts. There was no significant damaging effect of poly- and monodisperse AuNP solutions on the testes and accessory sexual glands of rats when administered for up to two weeks. The stimulating effect of administered orally a low dose of CeO2NP (1 mg/kg b.w.) on testosterone secretion and spermatogenesis, proliferative and secretory processes in the prostate of aging male rats was found. However, fertility of animals reduced in comparison with the control group due to immaturity of a part of the spermatozoa. The mechanisms of the stimulating or damaging effects of metal nanoparticles and their salts on normal and malignant tissues require further research in order to evaluate their therapeutic potential and toxicity.

Keywords
gold nanoparticles, cerium dioxide nanoparticles, reproductive system, aging, prostate cancer

Cite this paper
Reznikov A.G., Faliush O.A., Nosenko N.D., Sachynska O.V., Polyakova L.I., Limareva A.A., Perchyk I.G., Experimental evaluation of the influence of gold nanoparticles and cerium dioxide on normal and malignant cells and tissues , SCIREA Journal of Medicine. Volume 4, Issue 3, June 2020 | PP. 44-54.

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