Investigate the components and thermal behavior of milk thistle seed oil

Volume 5, Issue 1, February 2024     |     PP. 1-19      |     PDF (593 K)    |     Pub. Date: June 16, 2024
DOI: 10.54647/food550062    98 Downloads     1991 Views  


Zahra Amini, Department of Research and Science, Newsha Herbal Drink Company, Tehran, Iran
Adib Azizian, Department of Research and Science, Newsha Herbal Drink Company, Tehran, Iran
Ahmad Eyvazi, Department of Research and Science, Newsha Herbal Drink Company, Tehran, Iran

Milk thistle seed oil has recently been approved as a novel food resource. Despite its growing popularity, there is limited information on the constituents and properties of this oil. This study aims to compare the lipid composition, nutraceutical content, antioxidant activity, and thermal properties of milk thistle seed oils extracted through different methods (using hexane/ethanol or by cold press) from Iranian milk thistle seeds. The findings reveal that the extraction method does not significantly affect the fatty acid and triacylglycerol profiles. The main fatty acids identified are linoleic acid (45.83–46.41%) and oleic acid (30.12–30.59%), with oleic-linoleic-linoleic (OLL, ~20–21%) being the most prevalent triacylglycerol, followed by linoleic-linoleic-linoleic (LLL, ~18%), palmitic-oleic-linoleic (POL, ~15%), and palmitic-linoleic-linoleic (PLL, ~11%). Conversely, the extraction method significantly influences the minor component content and antioxidant activity of the oil. Ethanol-extracted oil contains less total vitamin E and sterols but more tocotrienols and exhibits stronger free radical scavenging capacity. Additionally, cold-pressed oil displays a more complex melting profile compared to solvent-extracted oil. These results are valuable for the quality assessment and industrial production of milk thistle seed oil.

Milk thistle seed oil , Thermal analysis , Components, Vitamin E

Cite this paper
Zahra Amini, Adib Azizian, Ahmad Eyvazi, Investigate the components and thermal behavior of milk thistle seed oil , SCIREA Journal of Food. Volume 5, Issue 1, February 2024 | PP. 1-19. 10.54647/food550062


[ 1 ] Ahmed, I. A. M., Al-Juhaimi, F. Y., Ozcan, M. M., Osman, M. A., Gassem, M. A., & Salih, H. A. A. (2019). Effects of cold-press and soxhlet extraction systems on antioxidant activity, total phenol contents, fatty acids, and tocopherol contents of walnut kernel oils. Journal of Oleo Science, 68, 167–173.
[ 2 ] Andrzejewska, J., Martinelli, T., & Sadowska, K. (2015). Silybum marianum: Non-medical exploitation of the species. Annals of Applied Biology, 167, 285–297.
[ 3 ] AOCS (1997). Official methods and recommended practices of the American oil chemists' society (5th ed.). Champaign III USA: AOCS Press.
[ 4 ] Azadmard-Damirchi, S., Habibi-Nodeh, F., Hesari, J., Nemati, M., & Achachlouei, B. F. (2010). Effect of pretreatment with microwaves on oxidative stability and nutraceuticals content of oil from rapeseed. Food Chemistry, 121, 1211–1215.
[ 5 ] Baumler, E. R., Carrin, M. E., & Carelli, A. A. (2016). Extraction of sunflower oil using ethanol as solvent. Journal of Food Engineering, 178, 190–197.
[ 6 ] Ben Rahal, N., Barba, F. J., Barth, D., & Chevalot, I. (2015). Supercritical CO2 extraction of oil, fatty acids and flavonolignans from milk thistle seeds: Evaluation of their antioxidant and cytotoxic activities in Caco-2 cells. Food and Chemical Toxicology, 83, 275–282.
[ 7 ] Benzie, I. F. F., & Strain, J. J. (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15–27.
[ 8 ] Chen, H., Qiu, S., Gan, J., Li, Z., Nirasawa, S., & Yin, L. (2016). New insights into the antioxidant activity and components in crude oat oil and soybean oil. Journal of Food Science & Technology, 53, 808–815.
[ 9 ] Dabbour, I. R., Al-Ismail, K. M., Takruri, H. R., & Azzeh, F. S. (2014). Chemical characteristics and antioxidant content properties of cold pressed seed oil of wild milk thistle plant grown in Jordan. Pakistan Journal of Nutrition, 13, 67–78.
[ 10 ] Fanoudi, S., Alavi, M. S., Karimi, G., & Hosseinzadeh, H. (2018). Milk thistle (Silybum marianum) as an antidote or a protective agent against natural or chemical toxicities: A review. Drug and Chemical Toxicology, 1–15.
[ 11 ] Fathi-Achachlouei, B., & Azadmard-Damirchi, S. (2009). Milk thistle seed oil constituents from different varieties grown in Iran. Journal of the American Oil Chemists Society, 86, 643–649.
[ 12 ] Fathi-Achachlouei, B., Azadmard-Damirchi, S., Zahedi, Y., & Shaddel, R. (2019). Microwave pretreatment as a promising strategy for increment of nutraceutical content and extraction yield of oil from milk thistle seed. Industrial Crops and Products, 128, 527–533.
[ 13 ] Gai, Q. Y., Jiao, J., Mu, P. S., Wang, W., Luo, M., Li, C. Y., & Fu, Y. J. (2013). Microwaveassisted aqueous enzymatic extraction of oil from Isatis indigotica seeds and its evaluation of physicochemical properties, fatty acid compositions and antioxidant activities. Industrial Crops and Products, 45, 303–311.
[ 14 ] Gao, P., Liu, R., Jin, Q., & Wang, X. (2019). Comparative study of chemical compositions and antioxidant capacities of oils obtained from two species of walnut: Juglans regia and Juglans sigillata. Food Chemistry, 279, 279–287.
[ 15 ] Hadolin, M., Skerget, M., Knez, Z., & Bauman, D. (2001). High pressure extraction of vitamin E-rich oil from Silybum marianum. Food Chemistry, 74, 355–364.
[ 16 ] Harrabi, S., Curtis, S., Hayet, F., & Mayer, P. M. (2016). Changes in the sterol compositions of milk thistle oil (Silybium marianum L.) during seed maturation. Grasas Y Aceites, 67, e123.
[ 17 ] Harrabi, S., Romdhane, H., Daassa, M., & Fellah, H. (2015). Fatty acid and triacylglycerol compositions of milk thistle seeds growing wild in Tunisia (Silybum marianum L.). Acta Alimentaria, 44, 304–310.
[ 18 ] Hassanein, M. M., Elshami, S. M., & Elmallah, M. H. (2003). Detailed studies on some lipids of Silybum marianum (L.) seed oil. Grasas y Aceites, 54, 397–402.
[ 19 ] Holčapek, M., Jandera, P., Zderadička, P., & Hruba, L. (2003). Characterization of triacylglycerol and diacylglycerol composition of plant oils using high-performance liquid chromatography–atmospheric pressure chemical ionization mass spectrometry. Journal of Chromatography A, 1010, 195–215.
[ 20 ] Kazazis, C. E., Evangelopoulos, A. A., Kollas, A., & Vallianou, N. G. (2014). The therapeutic potential of milk thistle in diabetes. The Review of Diabetic Studies, 11, 167–174.
[ 21 ] Lim, W. T., & Nyam, K. L. (2016). Characteristics and controlled release behaviour of microencapsulated kenaf seed oil during in-vitro digestion. Journal of Food Engineering, 182, 26–32.
[ 22 ] Li, F., Yang, L., Zhao, T., Zhao, J., Zou, Y., Zou, Y., et al. (2012). Optimization of enzymatic pretreatment for n-hexane extraction of oil from Silybum marianum seeds using response surface methodology. Food and Bioproducts Processing, 90, 87–94.
[ 23 ] Meddeb, W., Rezig, L., Abderrabba, M., Lizard, G., & Mejri, M. (2017). Tunisian milk thistle: An investigation of the chemical composition and the characterization of its cold-pressed seed oils. International Journal of Molecular Sciences, 18, e2582.
[ 24 ] Nyam, K. L., Tan, C. P., Che Man, Y. B., Lai, O. M., & Long, K. (2009a). Physicochemical properties of Kalahari melon seed oil following extractions using solvent and aqueous enzymatic methods. International Journal of Food Science and Technology, 44, 694–701.
[ 25 ] Nyam, K. L., Tan, C. P., Lai, O. M., Long, K., & Che Man, Y. B. (2009b). Physicochemical properties and bioactive compounds of selected seed oils. Lebensmittel-Wissenschaft und -Technologie- Food Science and Technology, 42, 1396–1403.
[ 26 ] Orsavova, J., Misurcova, L., Ambrozova, J. V., Vicha, R., & Mlcek, J. (2015). Fatty acids composition of vegetable oils and its contribution to dietary energy intake and dependence of cardiovascular mortality on dietary intake of fatty acids. International Journal of Molecular Sciences, 16, 12871–12890.
[ 27 ] Post-White, J., Ladas, E. J., & Kelly, K. M. (2007). Advances in the use of milk thistle (Silybum marianum). Integrative Cancer Therapies, 6, 104–109.
[ 28 ] Samaram, S., Mirhosseini, H., Tan, C. P., Ghazali, H. M., Bordbar, S., & Serjouie, A. (2015). Optimisation of ultrasound-assisted extraction of oil from papaya seed by response surface methodology: Oil recovery, radical scavenging antioxidant activity, and oxidation stability. Food Chemistry, 172, 7–17.
[ 29 ] Samyn, P., Schoukens, G., Vonck, L., Stanssens, D., & Abbeele, H. V. (2012). Quality of Brazilian vegetable oils evaluated by (modulated) differential scanning calorimetry. Journal of Thermal Analysis and Calorimetry, 110, 1353–1365.
[ 30 ] Shi, L. K., Zheng, L., Mao, J. H., Zhao, C. W., Huang, J. H., Liu, R. J., & Wang, X. G. (2018). Effects of the variety and oil extraction method on the quality, fatty acid composition and antioxidant capacity of Torreya grandis kernel oils. LWT-Food Science and Technology, 91, 398–405.
[ 31 ] Soleimani, V., Delghandi, P. S., Moallem, S. A., & Karimi, G. (2019). Safety and toxicity of silymarin, the major constituent of milk thistle extract: An updated review. Phytotherapy Research, 33, 1627–1638.
[ 32 ] Tajmohammadi, A., Razavi, B. M., & Hosseinzadeh, H. (2018). Silybum marianum (milk thistle) and its main constituent, silymarin, as a potential therapeutic plant in metabolic syndrome: A review. Phytotherapy Research, 32, 1933–1949.
[ 33 ] Tan, C. X., Chong, G. H., Hamzah, H., & Ghazali, H. M. (2018). Comparison of subcritical CO2 and ultrasound-assisted aqueous methods with the conventional solvent method in the extraction of avocado oil. The Journal of Supercritical Fluids, 135, 45–51.
[ 34 ] Tan, C. H., Xu, X. R., Shang, Y. Q., Fu, X. L., Xia, G. H., & Yang, H. (2014). A novel approach for the efficient extraction of silybin from milk thistle fruits. Pharmacognosy Magazine, 10, 536–540.
[ 35 ] Zhang, Z. S., Kang, Y. J., & Che, L. M. (2019). Composition and thermal characteristics of seed oil obtained from Chinese amaranth. LWT-Food Science and Technology, 111, 39–45.
[ 36 ] Zhang, Z. S., Liu, Y. L., & Che, L. M. (2018). Characterization of a new α-linolenic acidrich oil: Eucommia ulmoides seed oil. Journal of Food Science, 83, 617–623.